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Details

Stereochemistry ACHIRAL
Molecular Formula 3Na.O3PS
Molecular Weight 180.006
Optical Activity NONE
Defined Stereocenters 0 / 0
E/Z Centers 0
Charge 0

SHOW SMILES / InChI
Structure of SODIUM THIOPHOSPHATE

SMILES

[Na+].[Na+].[Na+].[O-]P([O-])([S-])=O

InChI

InChIKey=RIFYBBXGYKFBFC-UHFFFAOYSA-K
InChI=1S/3Na.H3O3PS/c;;;1-4(2,3)5/h;;;(H3,1,2,3,5)/q3*+1;/p-3

HIDE SMILES / InChI

Molecular Formula HO3PS
Molecular Weight 112.045
Charge -2
Count
MOL RATIO 1 MOL RATIO (average)
Stereochemistry ACHIRAL
Additional Stereochemistry No
Defined Stereocenters 0 / 0
E/Z Centers 0
Optical Activity NONE

Molecular Formula Na
Molecular Weight 22.9898
Charge 1
Count
MOL RATIO 3 MOL RATIO (average)
Stereochemistry ACHIRAL
Additional Stereochemistry No
Defined Stereocenters 0 / 0
E/Z Centers 0
Optical Activity NONE

Description

Sodium thiophosphate (or sodium monothiophosphate) a biochemical reagent, a specific inhibitor of protein tyrosine phosphatase and a competitive inhibitor of alkaline phosphatase. Thiophosphate was effective in inducing apoptosis in some leukemia cell lines including CEM and K562 and a lymphoma cell line, Raji. In addition, monothiophosphate entered intracellular nucleotide pools and served as an effective precursor for the phosphorylation of nuclear proteins in vivo.

Approval Year

Targets

Primary TargetPharmacologyConditionPotency
0.47 mM [IC50]

Conditions

ConditionModalityTargetsHighest PhaseProduct

PubMed

Sample Use Guides

In Vivo Use Guide
Unknown
Route of Administration: Unknown
In Vitro Use Guide
Thiophosphate (SPO(3)) was recently shown to promote cysteine insertion at Sec (selenocysteine)-encoding UGA codons during selenoprotein synthesis. The irreversible targeting by cDDP [cis-diamminedichloroplatinum(II) or cisplatin] of the Sec residue in TrxR1 (thioredoxin reductase 1) contributes to cDDP cytotoxicity. This effect could possibly be attenuated in cells expressing less reactive Sec-to-cysteine-substituted TrxR1 variants, or pronounced in cells with higher levels of Sec-containing TrxR1. To test this, there were supplemented cells with either SPO(3) or selenium and subsequently determined total as well as specific activities of cellular TrxR1, together with extent of drug-induced cell death. It was found that cDDP became less cytotoxic after incubation of A549 or HCT116 cells with lower SPO(3) concentrations (100-300 μM), whereas higher SPO(3) (>300 μM) had pronounced direct cytotoxicity. NIH 3T3 cells showed low basal TrxR1 activity and high susceptibility to SPO(3) cytotoxicity, or to glutathione depletion.
Substance Class Chemical
Record UNII
AN1017219I
Record Status Validated (UNII)
Record Version