In a model of hyperoxia-induced lung injury, SB-265610 was injected 1, 2, or 3 mg/kg (10, 20, or 30 μg) i.p. once daily.

Chemotaxis of isolated rat neutrophils was assessed using 48-well microchemotaxis chambers. Migration was allowed to continue for 2 h at 37°C, 5% CO2. Experiments were performed in the presence of 0 to 1000 nM SB-265610, with 100 nM CINC-1 in the lower chambers. Neutrophils were permitted to chemotax onto filters, after which the upper side was washed in phosphate-buffered saline and scraped. The lower side was stained using Wright-Giemsa. The stained filters were examined with a microscope at 100× magnification, digital images of the stained filters were obtained, and cells were counted from the printed images.