Stereochemistry | ACHIRAL |
Molecular Formula | C14H10O |
Molecular Weight | 194.2286 |
Optical Activity | NONE |
Defined Stereocenters | 0 / 0 |
E/Z Centers | 0 |
Charge | 0 |
SHOW SMILES / InChI
SMILES
OC1=CC2=C(C=CC=C2)C3=C1C=CC=C3
InChI
InChIKey=DZKIUEHLEXLYKM-UHFFFAOYSA-N
InChI=1S/C14H10O/c15-14-9-10-5-1-2-6-11(10)12-7-3-4-8-13(12)14/h1-9,15H
Molecular Formula | C14H10O |
Molecular Weight | 194.2286 |
Charge | 0 |
Count |
MOL RATIO
1 MOL RATIO (average) |
Stereochemistry | ACHIRAL |
Additional Stereochemistry | No |
Defined Stereocenters | 0 / 0 |
E/Z Centers | 0 |
Optical Activity | NONE |
9-Phenanthrol is a small molecule capable of modulating vascular and smooth muscle contractility through inhibition of the TRMP4 and Anoctamin-1 Ca(2+) channels. This mechanism of action has suggested implications for ischemic injuries to the heart and brain, as well as control over the excitability of vascular smooth muscle and urinary bladder smooth muscle.
Originator
Approval Year
PubMed
Sample Use Guides
Tetracycline-inducible HEK-293 cells were transfected to express the human TRPM4 channel and cultured in DMEM supplemented with 10% fetal bovine serum and 2 mM glutamine under a 5% CO2 atmosphere at 37 deg-C. The growth media was further supplemented with 5 micro-g/mL S-blasticidin and 0.4 mg/mL Zeocin. Cells were resuspended in media containing 1 micro-g/mL tetracycline 18 - 22 hours before experimentation. Whole-cell currents were recorded using the inside out configuration of the voltage patch-clamp technique. TRPM4 currents were monitored with seven doses of 9-phenanthrol between 10^-8 to 10^-4 M. At 10^-4 M 9-phenanthrol almost totally inhibited the channel current. The fitting of the dose-response curves with the Hill equation yielded an IC50 in the range of 2 × 10−5 m and showed no voltage dependence of the drug effect.