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Details

Stereochemistry ACHIRAL
Molecular Formula C27H25N2O7S2.Na
Molecular Weight 576.616
Optical Activity NONE
Defined Stereocenters 0 / 0
E/Z Centers 0
Charge 0

SHOW SMILES / InChI
Structure of ACID GREEN 50

SMILES

[Na+].CN(C)C1=CC=C(C=C1)C(=C2C=CC(C=C2)=[N+](C)C)C3=C(O)C(=CC4=CC(=CC=C34)S([O-])(=O)=O)S([O-])(=O)=O

InChI

InChIKey=WDPIZEKLJKBSOZ-UHFFFAOYSA-M
InChI=1S/C27H26N2O7S2.Na/c1-28(2)20-9-5-17(6-10-20)25(18-7-11-21(12-8-18)29(3)4)26-23-14-13-22(37(31,32)33)15-19(23)16-24(27(26)30)38(34,35)36;/h5-16H,1-4H3,(H2,31,32,33,34,35,36);/q;+1/p-1

HIDE SMILES / InChI

Molecular Formula Na
Molecular Weight 22.9898
Charge 1
Count
MOL RATIO 1 MOL RATIO (average)
Stereochemistry ACHIRAL
Additional Stereochemistry No
Defined Stereocenters 0 / 0
E/Z Centers 0
Optical Activity NONE

Molecular Formula C27H25N2O7S2
Molecular Weight 553.627
Charge -1
Count
MOL RATIO 1 MOL RATIO (average)
Stereochemistry ACHIRAL
Additional Stereochemistry No
Defined Stereocenters 0 / 0
E/Z Centers 0
Optical Activity NONE

Description

Acid green 50 (Lissamine Green B) is a synthetically produced organic acid dye with two aminophenyl groups. Acid green 50 is extensively used as a food dye and has a medical application as a staining agent. Due to its low toxicity and ability to stain dead and damaged cells the dye (in form of lissamine green strips) was approved as a tool for diagnosis of eye diseases associated with the damage of ocular surface epithelial cells.

Approval Year

Targets

Primary TargetPharmacologyConditionPotency

Conditions

ConditionModalityTargetsHighest PhaseProduct
Diagnostic
LISSAMINE GREEN

PubMed

Sample Use Guides

In Vivo Use Guide
For best viewing and patient comfort, acid green 50 (Lissamine Green) impregnated tip should be moistened with one or two drops of sterile irrigating or saline solution before application. Apply moistened tip to conjunctiva or fornix as required. It is recommended that the patient blink several times after application.
Route of Administration: Other
In Vitro Use Guide
Human corneal epithelial (HCE) cells were exposed to 1, 0.5, or 0.1% of acid green 50 (lissamine green) and the cells viability was studied. No observable staining of healthy HCE cells was noted with acid green 50.
Substance Class Chemical
Record UNII
9B7E8Y9D0X
Record Status Validated (UNII)
Record Version