3,5-di-tert-butyl- 4-hydroxybenzaldehyde is metabolites of a food additive butylated hydroxytoluene (BHT). It has less prominent radical-scavenging activity than butylated hydroxytoluene. As the anthropogenic pollutant, it was detected in human breast adipose tissues. 3,5-di-tert-butyl- 4-hydroxybenzaldehyde was found in essential oil of Sphaeranthus indicus and in the capsules of Asclepias physocarpa. It is used as a reagent in the synthesis of a series of quinolinone-chalcones, which have anti-parasitic activity and in the synthesis of thiazolo[3,2-b][1,2,4]triazole-6(5H)-one derivatives (a potent analgesic and anti-inflammatory agents).