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To explore the anti-cell proliferation mechanism of curcumol the changes of Jak2-STAT1/3 signal pathway-related molecules in synoviocytes were observed in vitro. In this study, the fibroblast-like synoviocytes (FLS) in patients with RA were collected and cultured. The following parameters were measured: cell proliferation (WST-1 assay in the presence of different concentrations of curcumol: 25, 50, 100, 200, 400, and 800 g/mL), cell cycles (fluorescence-activated cell sorting, FACS in the presence of different concentration of curcumol: 25, 50, 100 g/mL), STAT1 and STAT3 activities (electrophoretic mobility shift assay, EMSA), and the protein expressions of phosphorylated Jak2, STAT1, and STAT3 (Western blot). It was shown, that certain concentration of curcumol ranging from 25 g/mL to 100 g/mL could inhibit the RA-FLS proliferation and DNA synthesis induced by PDGF-BB in a dose-dependent manner in vitro. The fibroblast-like synoviocytes (FLS)