The 13 week oral toxicity study in rats revealed a NOEL of 100 mg/kg bw/day

D&C Green No. 5 activity was evaluated against human immunodeficiency virus type 1 (HIV-1) in primary human lymphocytes. The antiviral assays were conducted in mitogen stimulated human PBMC infected with HIV-1 (strain LAV). Stock solutions of the drugs were prepared in DMSO and diluted so that the final concentration of DMSO in the medium did not exceed 0.05%. The D&C Green No. 5 were added about 45 min after infection. Six days later when virus production was at a maximum, the supernatant was clarified and the virus concentrated by high speed centrifugation. The reverse transcriptase activity associated with the disrupted virus was determined. The virus infected control had about 3 x 105 decompositions per minute per ml of reverse transcriptase activity. The blank and uninfected cell control values were about 500 and 1200 dpm, respectively