In toxicology experiments, plumieride was fed to male rats orally at doses of (15 mg/rat/day) for the period of 60 days.

For anti-fungal experiment, 5 mg of of the plumieride was dissolved in 10 mL of 50% ethanol. The assay disc was impregnated with 2mL of the solution of compound prepared in 50% ethanol by repeated addition and evaporation of the solvent. For the control, discs were impregnated with 50% ethanol. The assay discs were transferred aseptically to the Petri plates containing Sabouraud dextrose agar medium. The plates were inoculated separately by the mycelial disc of the test dermatophytes, Epidermophyton floccosum and Microsporum gypseum. The plates were incubated at 28°1°C for 6 days and the colony diameter of the test dermatophytes of the treatment and control sets were measured on day 7 and the per cent inhibition of growth of test dermatophytes was calculated.