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Human colorectal carcinoma HCT-116 cells were grown in 12-well plates in McCoy’s 5A Modified Medium supplemented with fetal bovine serum (10% /volume) and penicillin/streptomycin (1% /volume). Cells incubated for approximately two days until 80% confluency was reached. After the desired confluency was reached, the medium was removed and the cells rinsed with phosphate buffered saline, and charged a combination of new media (0.375 mL) along with a volume of Cu2-xSe dispersion (0.125 mL, 157 mg/L) to give a solution concentration of 39 mg/L. Cells were incubated for 0.5 hr, 1 hr, 3 hr, and 6 hr at 37 Celsius degree. After the incubation the media was replaced to remove all unbound Cu2-xSe nanocrystals. The cells were exposed to a membrane permeability stain, Trypan blue, to assess cell death.