tablet twice a day of a K-712 (10 mg of oleoresin from Pseudowintera colorata at 30 percent concentration in Polygodial) for 4 weeks and then for the first 2 weeks of each month for a total of 6 months.

Mice beta-pancreatic insulinoma INS 1E cell line were used for activity evaluation. INS 1E cells (2 x 10^5/well) were seeded in 24-well plate and incubated in the same conditions of regular cell culture. Different polygodial concentrations (30, 300, 3000 nM, diluted in EtOH) stimulus were assessed for 1 h and 24 h of incubation in order to evaluate the acute and chronic effect of the compound, respectively. After growth and treatment with polygodial, cells were detached with trypsin and 1 mL of the PBS suspension and centrifuged. The cellular pellet was resuspended in 300 mkL of a 50 mkg/mL propidium iodide 0.1% hypotonic solution with sodium citrate 0.1% and Triton X-100 0.1%. Afterwards, samples were incubated for 30 min at 4 C and analyzed by flow cytometry in a FACScalibur system (BD Bioscience, San Jose, CA, USA).