Tricetin was administered to four rats at an oral dose of 100 mg which resulted in the urinary excretion of unchanged tricetin, accompanied by small amounts of 3,5-dihydroxyphenylpropionic acid.

Human MCF-7 breast cancer cells were maintained in monolayer cultures at 37 deg-C under a 5% CO2 atmosphere in DMEM supplemented with 10% FCS, 5 micro-g/mL insulin, 100 units/mL of penicillin G, 100 micro-g/mL of streptomycin, and 0.25 micro-g/mL of amphotericin B. Cells were plated in 96-well culture plates, and incubated for 24 hours prior to treatment with various concentrations of triceten and a further 48 hour incubation period. Inhibition of cell proliferation by tricetin was measured by XTT assay. Triceten inhibited the growth of MCF-7 cells in a dose-dependent manner with an IC50 value of 32.17 micro-M.