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Cytotoxicity study of human cancer cell lines (A549, SW480, MCF-7, HepG2, A2780, A2780/TAX, HCT-8, HCT-8/VCT, A549/CDDP, MCF-7/DOX, and NIH-3T3) of different tissues was determined using 96-well tissue culture plates. Sulforhodamine B (SRB) assay was performed to evaluate cell viability and to obtain the IC50 values. Cells were plated at 5000 cells/well. The cells were allowed to grow in carbon dioxide incubator (37 C, 5% CO2, 90% RH) for 24 h. Then test compounds (14, 10-Deacetylbaccatin III) in complete growth medium (100 L) were added to the wells in triplicate. The plates were further incubated for 48 h. The cell growth was stopped by gently layering trichloroacetic acid (50%, 50 L) on top of the medium in all the wells. The plates were incubated at 4 C for 1 h to fix the cells attached to the bottom of the wells. The liquid of all the wells was gently pipetted out and discarded. The plates were washed five times with distilled water to remove trichloroacetic acid, growth medium, low molecular weight metabolites, serum proteins etc. and air-dried. The plates were stained with SRB dye (0.4% in 1% acetic acid, 100 L) for 30 min. The plates were washed five times with 1% acetic acid and then air-dried. The adsorbed dye was dissolved in Tris base solution (150 L, 10 mM, pH 10.4) and plates were gently shaken for 1 h on an orbital shaker. The optical density (OD) was recorded on a TECAN infinite M200 pro multimode reader at 515 nm.