Oral dose between 100 to 200 mg

Rat synaptosomes were used for activity evaluation. Synaptosomes were preloaded with radiolabeled substrate in Krebs-phosphate buffer for 1 h (steady state). For release assays, 9 nM [3H]1-methyl-4-phenylpyridinium ( [3H]MPP+) was used as the radiolabeled substrate for DAT, whereas 5 nM [3H]5-HT was used as a substrate for SERT. All buffers used in the release assay methods contained 1 μM reserpine to block vesicular uptake of substrates. Release assays were initiated by adding 850 μL of preloaded synaptosomes to 150 μL of test drug (Methoxyphedrine). Release was terminated by vacuum filtration, and retained radioactivity was quantified by liquid scintillation counting. Effects of test drug concentrations were expressed as percentage of maximum release, with maximum release (i.e. 100% Emax) was defined as the release produced by 10 nM tyramine for DAT and NET assay conditions, and 100 nM tyramine for SERT assay conditions.