For treatment of peptic ulcers, ecabet sodium is indicated orally, 1 g b.i.d. For treatment of dry eye syndrome, topical administration was used (one drop in study eye 4 times daily for 90 days).

To measure bactericidal activity at pH 3.0, a liquid cultivation method was used. H. pylori NCTC 11637 was inoculated in 5 ml of brain heart infusion broth supplemented with 10% heat-inactivated fetal bovine serum and incubated at 378C for 48 h in an atmosphere of 5% O2–10% CO2–85% N2 with gyration. After rinsing with saline, bacterial cells were resuspended in saline to give about 109 CFU/ml. Reaction mixtures were prepared by adding 0.25 ml of an ecabet or BHA solution of citrate buffer supplemented with 10mM urea and incubated in a water bath at 37°C. Samples for viability (0.1 ml) were taken at 0, 15, 30, and 60 min. Viability was measured by the plate colony count technique. Ecabet showed concentration-dependent bactericidal effects at pHs 4.0 and 5.0, with MIC below 1 mg/ml.