Bisdequalinium was available in three dispensing forms: an irrigation solution, a working solution, and a medication paste. They contained 0.125 %, 0.5 %, and 0.48 % Bisdequalinium respectively.

L929 mouse fibroblasts cells were used for cytotoxicity evaluation. The culture medium used was minimal essential medium with Eagle's salts (Flow Laboratories, McLean, VA). Ten percent (vol/vol) fetal calf serum, 2 mM L-glutamine, and 2.2 mg of sodium bicarbonate per ml were added to the medium. Antibiotics at a concentration of 50 mkg of streptomycin and 100 IU of penicillin per ml were also added to the medium. The cells, media, procedures for radiochromium labeling of the target cells. In order to prepare desired concentrations of Bisdequalinium for the experiments, the stock Bisdequalinium solutions (0.125% Bisdequalinium) were diluted in the culture medium. One milliliter of the test solution was mixed with 1 ml of the labeled cell suspension in a test tube and incubated at 37C The test tubes were then centrifuged (500 x g), and the supernatants were counted in a gamma particle counter. The percentage of radiochromium release was calculated on the basis of the total amount of label incorporated in the target cells.