Unknown

Vero cell monolayers were seeded in 24-well plates. After 24 h (5% CO2 incubator, 37°C), cells were washed twice with phosphate buffer saline (PBS) and treated with 1.0 and 10 µg/mL of euparin in IM, corresponding to EC90and 10X EC90, respectively. After 7 h incubation at 37°C, the medium was removed; cell monolayers were washed three times with PBS and infected with 100 PFU/well of PV-1 in 0.1 mL of IM. Following 45 min of adsorption at 37°C, the virus inoculum was removed and PM was added. After 24 h, cells were fixed and stained and viral plaques were counted.