2-Amino-6-nitrobenzothiazole is an important intermediate product for the preparation of valuable azo dyestuffs. It has demonstrated in vitro antiprotozoal effect against Trichomonas vaginalis. 2-Amino-6-nitrobenzothiazole has been used: • as model analyte for voltammetric determination of electrochemically reducible organic substances • in the synthesis of 2-methyl-4-nitro-2H-pyrazole-3-carboxylic acid[2-(cyclohexanecarbonylamino)benzothiazol-6-yl]amide derivatives • in the preparation of push-pull nonlinear optical chromophores containing thiazole and benzothiazole acceptors • as a base in dye production by diazotation reaction.