Unknown

Human A2780 ovarian cancer cells were harvested and resuspended in growth medium (RPMI1640 supplemented with 10% fetal bovine serum and 2 mM L-glutamine). Cells were counted and distributed to a 96 well plate. Plates were incubated for 3 hours at 37 deg-C in a 5% CO2 atmosphere. β-peltatin-β-D-glucopyranoside (β-peltatin) was prepared in 50/50 H2O/DMSO add added to the wells in an appropriate series of concentrations. Plates were then incubated for 2 days. The percent inhibition of cell growth was determined by measuring the conversion of AlamarBlue by fluorescence. β-peltatin-β-D-glucopyranoside was found to have an IC50 of 4.9 microM with respect to the proliferation of human A278- ovarian cancer cells.