lethal dose < 5 mg/kg

Anococcygeus muscles were first contracted by a submaximal concentration of phenylephrine (0.1 μM). When the contractile response had reached a plateau, nicotine (1–100 μM) was added to the medium. After the response to nicotine was complete and had subsided, tissues were washed several times until tensions returned to resting level. The nicotine-induced noradrenergic contraction response was examined in the presence of the NO-stimulated soluble guanylyl cyclase inhibitor ODQ (oxadiazolo quinoxaline-1-one; 10 μM) in anococcygeus muscle. In the presence of coniin (1–100 μM), a sequential contraction-relaxation procedure with phenylephrine and nicotine applied. Coniine inhibited the noradrenergic contraction response both in 10 and 100 μM concentration (–logIC50  =  4.57 ± 0.12 (M)).