100 mg three times per day for 3 days

3T3-L1 cells were cultured in DMEM supplemented by 10% bovine calf serum. Two days after reaching confluency, differentiation was induced in DMEM supplemented with 10% fetal calf serum and dexamethasone (1 _M)/IBMX (0.5 mM)/ insulin (10 _g/ml). After 48 h, the medium was changed with only the addition of insulin for an additional 2 d. Thereafter, the cells were grown in DMEM supplemented with 10% fetal calf serum. Alternatively, cells were also differentiated with BRL 49653 (25 nM) and insulin (5 mkg/ml). SR-202 or vehicle (H2O) was added 24 h before induction of differentiation. Medium was replenished with ligands every 2 d. Adipogenesis was determined by the staining of lipids with Oil Red O and by measuring the expression of adipocyte markers.