For tick control in beef cattle applied topically as a pour-on for use at single dose levels of 1.5 and 2.5 mg/kg bw with a possible additional treatment after 3 to 6 months.

Human Bladder carcinoma cell lines RT112 and RT4 were used for activity evaluation. 7 x 10^3 cells per well were plated in 96-well plates and allowed to attach for 24 hr before adding testing chemicals. Cells were treated with testing chemicals for 24, 48, and 72 hr. After treatment, 10 μl 5 mg/ml 3-(4,5-methylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) was added and further incubated for 4 hr. The medium was then discarded and the precipitate dissolved in DMSO. The absorbance was measured at 570 nm with a Synergy 2 microplate reader (Bio-Tek Instruments, Inc., Winooski, VT, USA) according to the standard protocol.