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Description

BHA (E 320) is a synthetic antioxidant authorized as a food additive. BHA is a mixture of two isomers: 2-tert-butyl-4-hydroxyanisole and 3-tert-butyl-4-hydroxyanisole. BHA is known to have various beneficial activities such as antioxidant activity, anti-inflammatory effects, and anticancer potential although some reports suggested that this reagent induces cytotoxicity. In general, the majority of the genotoxicity studies indicate a lack of potential for BHA to induce point mutations or to interact with or damage DNA. It was banned in Japan in 1958, and it was recommended that it be banned in the UK, however, due to industry pressure it was not. The antioxidant properties of 3-tert-butyl-4-hydroxyanisole are achieved via inhibition of ROS generation. The experiments on mice have shown that pretreatment with compound prevented apoptotic cell death induced by oxidative stress in mouse hepatocytes that may be beneficial for treating liver diseases caused by oxidative stress.

Approval Year

Targets

Targets

Primary TargetPharmacologyConditionPotency
PubMed

PubMed

TitleDatePubMed
Estrogenic activity of phenolic additives determined by an in vitro yeast bioassay.
2001 Feb
Prediction of estrogen receptor agonists and characterization of associated molecular descriptors by statistical learning methods.
2006 Nov
[Expression changes of Notch-related genes during the differentiation of human mesenchymal stem cells into neurons].
2007 Jun 25
Development and evaluation of an electrochemical method for studying reactive phase-I metabolites: correlation to in vitro drug metabolism.
2007 May
Comparative study of the alkyl and peroxy radical-scavenging activity of 2-t-butyl-4-methoxyphenol (BHA) and its dimer, and their theoretical parameters.
2008 May-Jun
Sorption behavior of a synthetic antioxidant, polycyclic musk, and an organophosphate insecticide in wastewater sludge.
2009
Comparison of volatile profiles of nine litchi (Litchi chinensis Sonn.) cultivars from Southern China.
2009 Oct 28
Cameroonian medicinal plants: pharmacology and derived natural products.
2010
Determination of antioxidants in new and used lubricant oils by headspace-programmed temperature vaporization-gas chromatography-mass spectrometry.
2010 Dec
Radical-scavenging activity and cytotoxicity of p-methoxyphenol and p-cresol dimers.
2010 Feb 26
Potential involvement of F0F1-ATP(synth)ase and reactive oxygen species in apoptosis induction by the antineoplastic agent erucylphosphohomocholine in glioblastoma cell lines : a mechanism for induction of apoptosis via the 18 kDa mitochondrial translocator protein.
2010 Jul
Antimicrobial and antioxidant effects of phenolic constituents from Klainedoxa gabonensis.
2010 Oct
Patents

Sample Use Guides

In Vivo Use Guide
BHA (E 320) is a synthetic antioxidant authorised as a food additive. BHA is a mixture of two isomers with full chemical names 2-tert-butyl-4-hydroxyanisole and 3-tert-butyl-4-hydroxyanisole.
Route of Administration: Oral
In Vitro Use Guide
It was examined the effect of BHA against hydrogen peroxide (H2O2)-induced apoptosis in primary cultured mouse hepatocytes. Butylated hydroxyanisole (BHA; 3-tert-butyl 4-hydroxyanisole) is one of the most widely used synthetic phenolic compounds. To determine the effect of BHA on decreased cell viability caused by H2O2, cells were pretreated with various concentrations (0~10 µM) of BHA for 30 min and then treated with 1,000 µM H2O2. BHA prevented the decreases in cell viability caused by H2O2. These results indicate that BHA pretreatment protects primary cultured mouse hepatocytes from H2O2-induced apoptosis.
Name Type Language
BUTYLATED HYDROXYANISOLE
FHFI   HSDB   II   MART.   MI   NF   VANDF   WHO-DD   WHO-IP  
Common Name English
BUTYLATED HYDROXYANISOLE [HSDB]
Common Name English
EMBANOX
Common Name English
BUTYLHYDROXYANISOLE
Common Name English
BUTYLATED HYDROXYANISOLE [FHFI]
Common Name English
BUTYLATED HYDROXYANISOLE [NF]
Common Name English
INS NO.320
Code English
BUTYLATED HYDROXYANISOLE [VANDF]
Common Name English
BUTYLATED HYDROXYANISOLE [WHO-DD]
Common Name English
BUTYLATED HYDROXYANISOLE [MART.]
Common Name English
BHA [INCI]
Common Name English
BUTYLATED HYDROXYANISOLE [II]
Common Name English
BUTYLHYDROXYANISOLUM [WHO-IP LATIN]
Common Name English
TERT-BUTYL-4-METHOXYPHENOL
Common Name English
(1,1-DIMETHYLETHYL)-4-METHOXYPHENOL
Common Name English
3-TERTIARY-BUTYL-4-HYDROXYANISOLE
Common Name English
BUTYLATED HYDROXYANISOLE [MI]
Common Name English
FEMA NO. 2183
Code English
2(3)-TERT-BUTYL-4-HYDROXYANISOLE
Common Name English
ANTIOXYNE B
Common Name English
BHA [FCC]
Common Name English
TERT-BUTYL-P-HYDROXYANISOLE
Common Name English
BUTYLATED HYDROXYANISOLE [WHO-IP]
Common Name English
PHENOL, (1,1-DIMETHYLETHYL)-4-METHOXY-
Common Name English
INS-320
Code English
BHA
FCC   INCI  
INCI  
Official Name English
E-320
Code English
Classification Tree Code System Code
JECFA EVALUATION INS-320
Created by admin on Tue Mar 06 12:04:07 UTC 2018 , Edited by admin on Tue Mar 06 12:04:07 UTC 2018
CODEX ALIMENTARIUS (GSFA) INS-320
Created by admin on Tue Mar 06 12:04:07 UTC 2018 , Edited by admin on Tue Mar 06 12:04:07 UTC 2018
CFR 21 CFR 171.110
Created by admin on Tue Mar 06 12:04:07 UTC 2018 , Edited by admin on Tue Mar 06 12:04:07 UTC 2018
Code System Code Type Description
MERCK INDEX
M2819
Created by admin on Tue Mar 06 12:04:07 UTC 2018 , Edited by admin on Tue Mar 06 12:04:07 UTC 2018
PRIMARY Merck Index
NCI_THESAURUS
C44347
Created by admin on Tue Mar 06 12:04:07 UTC 2018 , Edited by admin on Tue Mar 06 12:04:07 UTC 2018
PRIMARY
RXCUI
1362900
Created by admin on Tue Mar 06 12:04:07 UTC 2018 , Edited by admin on Tue Mar 06 12:04:07 UTC 2018
PRIMARY RxNorm
EVMPD
SUB11764MIG
Created by admin on Tue Mar 06 12:04:07 UTC 2018 , Edited by admin on Tue Mar 06 12:04:07 UTC 2018
PRIMARY
CAS
25013-16-5
Created by admin on Tue Mar 06 12:04:07 UTC 2018 , Edited by admin on Tue Mar 06 12:04:07 UTC 2018
PRIMARY
JECFA MONOGRAPH
INS-320
Created by admin on Tue Mar 06 12:04:07 UTC 2018 , Edited by admin on Tue Mar 06 12:04:07 UTC 2018
PRIMARY
EPA CompTox
25013-16-5
Created by admin on Tue Mar 06 12:04:07 UTC 2018 , Edited by admin on Tue Mar 06 12:04:07 UTC 2018
PRIMARY
ECHA (EC/EINECS)
246-563-8
Created by admin on Tue Mar 06 12:04:07 UTC 2018 , Edited by admin on Tue Mar 06 12:04:07 UTC 2018
PRIMARY
MESH
D002083
Created by admin on Tue Mar 06 12:04:07 UTC 2018 , Edited by admin on Tue Mar 06 12:04:07 UTC 2018
PRIMARY
WHO INTERNATIONAL PHARMACOPEIA
BUTYLATED HYDROXYANISOLE
Created by admin on Tue Mar 06 12:04:07 UTC 2018 , Edited by admin on Tue Mar 06 12:04:07 UTC 2018
PRIMARY Description: A white or almost white, crystalline powder or a yellowish white solid; odour, faint and characteristic. Solubility: Practically insoluble in water; freely soluble in ethanol (~750 g/l) TS, ether R, propylene glycol R, and arachis oil R; dissolves in solutions of alkali hydroxides. Category: Antioxidant. Storage: Butylated hydroxyanisole should be kept in a well-closed container, protected from light. Definition: Butylated hydroxyanisole contains a variable amount of 3-tert-butyl-4-methoxyphenol.
WIKIPEDIA
BUTYLATED HYDROXYANISOLE
Created by admin on Tue Mar 06 12:04:07 UTC 2018 , Edited by admin on Tue Mar 06 12:04:07 UTC 2018
PRIMARY
HSDB
25013-16-5
Created by admin on Tue Mar 06 12:04:07 UTC 2018 , Edited by admin on Tue Mar 06 12:04:07 UTC 2018
PRIMARY
ChEMBL
CHEMBL192451
Created by admin on Tue Mar 06 12:04:07 UTC 2018 , Edited by admin on Tue Mar 06 12:04:07 UTC 2018
PRIMARY
All of the following components must be present:
Definition References