Swiss albino mice (25–30g) were treated with Rosmanol (50, 150 and 200 mg/kg).

Antimicrobial activity was determined against Gram-positive (Bacillus subtilis ATCC 6051, B. cereus ATCC 21772, Staphylococcus aureus ATCC 6538, S. epidermidis ATCC 14990, Enterococcus faecalis ATCC 29212, Mycobacterium smegmatis ATCC 19420) and Gram-negative (Escherichia coli ATCC 9637, Proteus mirabilis CECT 170, Pseudomonas aeruginosa AK 958, Salmonella sp CECT 4569) bacteria and the yeast Candida albicans CECT 1039. The bacteria cultures were developed in nutrient broth (NB) or brain heart infusion broth (for E. faecalis, and M. smegmatis containing 0.06% Tween 80), and the yeast was cultured in Sabouraud liquid medium at 37 C. All media were purchased from Oxoid. The minimal inhibitory concentration (MIC) was determined for each compound in triplicate, by the broth microdilution method The compounds (Rosmanol) were dissolved in DMSO, and wells with the same proportions of DMSO were used as controls and never exceeded 1% (v/v). The starting microorganism concentration was approximately (1-5) x 10^5 cfu/mL, and growth was monitored by measuring the increase in optical density at 550 nm (OD550) with a microplate reader (Multiskan Plus II). All wells with no visible growth after 24 h, except for M. smegmatis (48 h), in a rotatory shaker at 37 C were subcultured by transferring in duplicate (0.1 mL) to nutrient, brain heart infusion, or Sabouraud agar plates. After overnight incubation, colony counts were performed and the MIC was defined as the lowest concentration of compound at which growth was inhibited after 24 h of incubation.