Mice were induced to seizures by intravenous injection of NMDLA 150 mg/kg.

Tissue slices (0.35 mm thick) from the guinea pig cerebral cortex were prepared and incubated for 40 min in control media contained (mM): NaCl, 124 mM; KCl, 5 mM; KH2PO4, 1.2 mM; MgSO4, 1.2 mM; CaCl2, 2.4mM; NaHCO3, 26 mM and glucose, 10 mM; gassed with O2/CO2 (95:5) at 37°C. The slices were then incubated in control medium or in media containing glutamate or stereoisomers of N-methyl-aspartate. Tissues were also incubated in the same media with 5 mM [1-13C] glucose for various time periods. At the end of the incubation periods, the slices were rapidly removed by filtration, washed, homogenized, and centrifuged. Pellets were kept for protein determination. The supernatant was neutralized and dried by rotary-film evaporation. High concentrations of NMDLA (200 micro-M) resulted in decreases in PCr and ATP greater than NMDA (10 - 400 micro-M) alone. This result suggests that NMLA may be contributing more to tissue damage than NMDA, and was confirmed when tissues were exposed to NMLA (400 micro-M) alone. There was a significant increase in lactate and decrease in NAA within 30 min of exposure to 10 micro-M NMDA when Mg+ was absent from solution, but at longer times of exposure ( > 1hr) or higher concentrations of NMDA (400 micro-M these changes were also significant in the presence of Mg. The effects of the stereoisomers (NMDLA and NMLA; 20 - 800 micro-M) on lactate and NAA were essentially similar to those of NMDA itself.