1% ethanol solution of ethyl glucoside (alpha anomer) was applied on the dorsal surface of the mice, once a day, 5 times a week, for 4 weeks.

In a cell viability assay, normal human dermal fibroblasts were seeded at 2.0 × 10(4) cells/well into a 96-well culture plate containing 0.1 mL/well of low glucose Dulbecco’s modified Eagle’s medium supplemented with 10% (v/v) fetal bovine serum and were cultured for 24 h. After removing this medium, DMEM containing 1% (v/v) FBS with various concentrations of ethyl glucoside (alpha anomer) from 0.048 to 480 uM was added, and the culture was continued for 72 h. In MTT assay, cells were seeded at 5.0 × 10(3) cells/well into a 24-well culture plate containing 1 mL/well of DMEM with 10% (v/v) FBS and cultured for 24 h. After removing the medium, DMEM containing 10% (v/v) FBS with various concentrations of α-EG (0.048–4.8 μM) was added, and the cells were cultured for another 7 days. At 0.48 uM ethyl glucoside was found to increase the proliferation of normal human dermal fibroblasts by 121.0%, and the amount of collagen I produced by cells increased by 159.6%