Male Wistar rats were trained in a one-trial-learning passive avoidance task. Ethylestrenol (1 ng, 10 ng, 100 ng, 1000 ng, or 10 mkg) was given immediately after the training trial or 1 h before the first retention test. Retention tests were given both 24 h and 48 h after acquisition. Ethylestrenol at all doses improved retention under the low footshock conditions. In groups trained with the higher footshock, the steroid-treated groups performed no better than the vehicle controls.

Yeast from mid-log phase growth was diluted to OD600 = 1.0 in selective medium. Diluted yeast cultures were treated with steroid concentrations ranging from 0.01 nM to 10 mM (in a final volume of 5mkl). Following incubation yeast was lysed and assayed for beta-galactosidase activity.